| Stationary and mobile phases |
| partition coefficient and capacity factor (K and k') |
| Column efficiency and what affects it |
| Resolution |
| Programming |
| Quantitation and calibration methods |
| Understand and explain the basis of chromatographic separations |
| Know how changes in phases, column size or packing, etc. will affect the separation |
| Understand the principles governing column programming (either temperature or mobile phase) and when and why it is needed |
| Be able to carry out calculations to calibrate a chromatographic system and quantitate analytes |
Textbook chapter on Chromatography
Some overheads